Journal: iScience

Article Title: Alphaherpesvirus manipulates retinoic acid metabolism for optimal replication

doi: 10.1016/j.isci.2024.110144

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-ABCG1 , Proteintech , Cat# 13578-1-AP, RRID: AB_2220174.

Techniques: Recombinant, Transfection, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Software

Journal: Frontiers in Pharmacology

Article Title: Diterpenoids inhibit ox-LDL-induced foam cell formation in RAW264.7 cells by promoting ABCA1 mediated cholesterol efflux

doi: 10.3389/fphar.2023.1066758

Figure Lengend Snippet: Primers for real-time quantitative PCR.

Article Snippet: Rabbit polyclonal antibodies against ABCG1 (Cat No. 13578-1-AP), PPAR gamma (Cat No. 16643-1-AP), and NR1H3 (LXR alpha) (Cat No. 14351-1-AP) were purchased from Proteintech (Rosemont, IL, United States).

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Diterpenoids inhibit ox-LDL-induced foam cell formation in RAW264.7 cells by promoting ABCA1 mediated cholesterol efflux

doi: 10.3389/fphar.2023.1066758

Figure Lengend Snippet: Diterpenoids increase ABCA1 and ABCG1 expression. RAW264.7 macrophages were treated with 15 μmol/L indicated drugs and chemical compounds for 24 h. Cell lysates were collected, and the protein levels of PPARγ (A) and LXRα (B) were determined by western blotting. Corresponding quantitative results are shown as the mean ± SEM from three independent experiments (C,D) . Cellular RNA was collected for quantitative real-time PCR (qRT‒PCR) analysis. Data are shown as the mean ± SEM from three independent experiments (E,F) . Significance is presented as * p < .05 and ** p < .01 versus the vehicle (DMSO) control.

Article Snippet: Rabbit polyclonal antibodies against ABCG1 (Cat No. 13578-1-AP), PPAR gamma (Cat No. 16643-1-AP), and NR1H3 (LXR alpha) (Cat No. 14351-1-AP) were purchased from Proteintech (Rosemont, IL, United States).

Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Control

Journal: Frontiers in Pharmacology

Article Title: Diterpenoids inhibit ox-LDL-induced foam cell formation in RAW264.7 cells by promoting ABCA1 mediated cholesterol efflux

doi: 10.3389/fphar.2023.1066758

Figure Lengend Snippet: Treatment with diterpenoids increases the mRNA expression of ABCA1, LXRα, and PPARγ in ox-LDL-induced macrophage foam cells. RAW264.7 macrophages were treated with 15 μmol/L indicated compounds and 80 μg/mL ox-LDL for 24 h. Total RNA was extracted from the cells for quantitative real-time PCR (qRT‒PCR) analysis of the mRNA levels of ABCA1 (A) , ABCG1 (B) , LXRα (C) and PPARγ (D) . Data are shown as the mean ± SEM from three independent experiments. Significance is presented as * p < .05, ** p < .01 versus the vehicle (DMSO) group and # p < .05, ## p < .01 versus the control group.

Article Snippet: Rabbit polyclonal antibodies against ABCG1 (Cat No. 13578-1-AP), PPAR gamma (Cat No. 16643-1-AP), and NR1H3 (LXR alpha) (Cat No. 14351-1-AP) were purchased from Proteintech (Rosemont, IL, United States).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control